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KMID : 0368419910340030223
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Journal of Plant Biology
1991 Volume.34 No. 3 p.223 ~ p.228
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Induction and Free Amino Acid Analysis of Cellus from Canavalia lineata Leaf
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Abstract
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The callus of Canavalia lineata was induced from leaf tissues in MS medium supplemented with 10^-5 M kinetin and 10^-6 M IAA and was subcultured in Miller´s medium supplemented with 10^-5 M BAP and 10^-6 M 2,4-D. When free amino acids of callus were analysed by HPLC, canavanine was not detected in the callus cultured either in the dark or light. But exogenously supplied canavanine was accumulated or consumned in the callus of Canavalia lineata.
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